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Cholesterol bound to hemoglobin in normal human erythrocytes: a new form of
cholesterol in circulation? |
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Clin Biochem 2004 Jan;37(1):22-6 (ISSN: 0009-9120)
Nikolic M; Stanic D; Antonijevic N; Niketic V [Find other articles with
these Authors]
Department of Chemistry, University of Belgrade, Belgrade, SCG, Yugoslavia.
OBJECTIVE: To study lipid fraction that is occasionally observed in red
blood cell (RBC) hemolysate (supernatants from which membranes were
separated). STUDY DESIGN: Plasma lipid profiles, cholesterol (Ch) and
phospholipids (PL) in intact RBCs, RBC membranes and hemolysates were
examined in young healthy male population in winter and summer. RESULTS: The
RBC Ch and PL content was significantly higher than in membranes, both in
winter and summer. The "excess" of cholesterol (associated with
phospholipid) was bound to hemoglobin yielding Hb-lipid adduct (Hb-Ch), the
pools in the RBC membrane remaining virtually unaltered. Levels of
hemoglobin-lipid complex (Hb-Ch), which were significantly higher in winter
than in summer (30% and 19% of the total Hb, respectively), positively
correlated with plasma HDL cholesterol levels. CONCLUSION: To our knowledge,
this is the first demonstration of cholesterol binding to Hb. The results
suggest influence of plasma lipoprotein metabolism on the formation of
Hb-Ch.
Language: English
MEDLINE Indexing Date: 200312
Publication Type: Owner: NLM; Status: In-Process
Publication Type: Journal Article
PMID: 0014675558
Journal Code: IM
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Cholesterol
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